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Literature summary for 3.1.6.21 extracted from

  • Hagelueken, G.; Adams, T.M.; Wiehlmann, L.; Widow, U.; Kolmar, H.; Tuemmler, B.; Heinz, D.W.; Schubert, W.D.
    The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases (2006), Proc. Natl. Acad. Sci. USA, 103, 7631-7636 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme and complexes with substrate analog and products, hanging drop vapor diffusion method, using 12% (w/v) PEG 4000, 10% isopropanol, 200 mM LiCl, 100 mM sodium citrate, pH 6.0 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 SDS at pH 7.5 and 25°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I5I9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Mono Q column chromatography and Superdex 200 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-decane sulfonic acid + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
decyl sulfate + H2O
-
 Pseudomonas aeruginosa decanol + sulfate
-
 ?
hexyl sulfate + H2O
-
 Pseudomonas aeruginosa hexanol + sulfate
-
 ?
additional information D-glucosamine 2-sulfate, D-glucose 3-sulfate, 4-nitrocatecholsulfate and 4-nitrophenylsulfate are not cleaved by the enzyme Pseudomonas aeruginosa ?
-
-
octyl sulfate + H2O
-
 Pseudomonas aeruginosa octanol + sulfate
-
 ?
SDS + H2O
-
 Pseudomonas aeruginosa 1-dodecanol + sulfate + Na+
-
 ?

Synonyms

Synonyms Comment Organism
PA0740
-
 Pseudomonas aeruginosa
sdsA1
-
 Pseudomonas aeruginosa